In comic book canon (not counting movies or TV shows), Batman's most defining character trait is his absolute refusal to kill under any circumstances. He not only refuses to kill in self-defense, but he even refuses to kill to protect his adoptive children! The most famous example of that has got to be Jason Todd, the main antagonist of the movie "Batman: Under the Red Hood."
In the rare event Batman is forced to explain his reasoning behind this logic, he'll usually that the reason is because he feels that, once he allows himself that liberty, even in circumstances where it might be justified in hindsight (such as self-defense), he'll just become part of the same problem that he's trying to stop.
But I honestly just don't see the logic.
That might be fine, if it were just Batman's own point of view and it were never anything beyond that. For example, in The Dark Knight, the Joker postulates that, when people are put in peril, they will always commit horrible atrocities to save themselves. But that's clearly isn't the movie's point of view; it's clearly just The Joker's tainted view on the world. The movie repeatedly proves him wrong, the biggest example of which is when the two ships are about to sink, and the inhabitants of those ships actually help each other out rather than kill each other.
But for Batman's absolute refusal to kill, the stories where this stance is tested consistently affirm Batman's ideals. Like, for example, in "Batman: Under the Red Hood." As this guy explains at 15:52 of this video.
https://www.youtube.com/watch?v=9CG1bapLTHE
"This story validates Batman's ideals by having Jason try to shoot him ... killing is the easy thing to do, and Jason's proven that; he's killed a lot, now, and as soon as things don't go his way, he aims his gun at his old mentor and friend, instead of the psychopath who was really responsible for his death. Jason wanted to control crime, but he's lost it; he's almost as whacked out as the Joker."
The DC universe seems to take the stance that killing, even justifiable killing, has a similar effect on your brain as cannibalism. Cannibalism has been scientifically proven in the real world to slowly rot away the cannibal's brain. If you do it enough, you slowly become mentally unstable and even a psychopath. This is all 100% scientifically documented.
But the DC universe not only takes a similar stance on killing, but actually takes it one step further. If Batman's philosophy is to be believed, killing has an immediate effect on one's psyche, not a gradual effect. The minute Batman takes a life - just one life - for any reason, even in the most justified of circumstances, then his "psychopath" switch has been flipped, and it's only a matter of time before he goes on mass killing sprees for its own sake, just to satisfy his own insatiable bloodlust, much like the comic book equivalent of The Mask (not to be confused with the film adaptation starring Jim Carey).
If this were scientifically sound, then how do you explain the millions of people in the real world who kill in self-defense (e.g. because a burglar is breaking into his home, or because he's a soldier in a war), and then go on to commit precisely zero murders from that point on for the rest of their lives?
Sure, this is a comic book world, so you may argue that we can suspend out disbelief. But this isn't just escapism. The Batman comics clearly want to teach a moral lesson with these types of stories. We're meant to take Batman's refusal to kill to heart, and strive to achieve this ideal in our daily lives to the greatest extent practicable.
This isn't like Power Rangers, which is 100% escapism. We don't have to worry about the moral implications of killing people because the rangers only fight monsters, and the Rangers are rarely put in a position where they have to compromise their morals. This is in stark contrast to Super Sentai, who often do have to do morally ambiguous things, like in this episode where the Zyurangers are forced to kill an innocent victim of Bandora's deception for the greater good: http://www.jatoku.com/kyoryu-sentai-...rranean-beast/
But Batman's refusal to kill is supposed to be a moral lesson we should all take to heart ... which means that, if it doesn't hold up to logical or scientific scrutiny, it falls flat on its face.