Partly, yes. According to Mark Millar's Marvel Knights Spider-Man #1-12, Osborn was part of a consortium of crooked corporate-types and politicians who were concerned that the rise of superpowered do-gooders would be a threat to their wealth and status, either by actively fighting against and exposing their corruption and criminality or just putting their abilities to use genuinely improving human society. To keep said superpowered do-gooders "occupied," that consortium created many of the early supervillains that first menaced the Marvel Universe --- though they weren't expecting Norman Osborn to turn himself into a supervillain.
That said, I do like the idea posited by some here that nearly every attempt at human augmentation or enhancement in the Marvel Universe, successful or otherwise, was ultimately the product of Weapon Plus working out how to create the perfect (or at least most effective) mutant-killing human bioweapon through repeated trial and error. I could also see the likes of Power Broker and Norman Osborn (and his successors/fellow Goblins) easily explainable as having broken away from Weapon Plus's overarching plan to strike out on their own, thinking they were better off using the human augmentation/enhancement tech to turn a profit for themselves rather than "wasting" it on a quixotic effort to rid the world of mutants. As long as I'm at it, though, it could be explained or retconned that the spider that bit Peter Parker all those years ago was a product of Weapon Plus experimentation as well, given that they did use animal test subjects at one point. Oh, and the chemical spills that blinded young Matt Murdock while amplifying his other senses and put Jessica Jones in a coma from which she woke up with superpowers? We never knew exactly what those chemicals were or what was in them, so it could easily be explained or retconned that Weapon Plus or a spinoff was tinkering with experimental chemical formulas to augment human senses and/or physical capabilities and Matt and Jessica were the unwitting beneficiaries of those experiments due to their accidents.
I could even mention the Spider-Women, as per the poster that suggested Jessica Drew's origin being tied to Weapon Plus via the High Evolutionary and his tinkering with animal DNA --- and raise Julia Carpenter, who was explicitly meant to be a spider-powered super-soldier or super-agent answerable to the U.S. government via the Commission on Superhuman Activities. Who's to say that wasn't a Weapon Plus branch or splinter organization, too, especially given the existence of Deathweb, who were subjected to the same experiments as Julia, but broke bad? Frankly, the possibilities are endless at this point; the entire Marvel Universe, heroes and villains alike, could ultimately trace its origins back to Weapon Plus in some form or other, and imagine how that would impact the X-Men, knowing that almost all the non-mutant superheroes (and supervillains) were deliberately created or intended as a counterweight to their very existence.