I'd love to start a game as Ben as the Scarlet Spider with Kaine as the main villain in his OG costume. Later down the road, maybe at the very end of the game, Ben would become the new Spider-Man (in his classic Spider-Ben suit) and Kaine the new Scarlet Spider (making amends with his past and all that stuff). Spider-Verse in form of a DLC, it's original story in comics is very flat so it's not worthy of being full blown sequel.
Venom as a second villain would be nice. Ben beat the **** out of him in their first fight. Impact Webbing is such a cool thing to have.
Oh, and no Spider-Gwen. She's overrated af.
edit: And I want Black Cat DLC. But not like that joke from Insomniac. I want to play as Felicia through all DLC's mission. Batman Arkham style.