It's a really big battle between the two sides.
Superman
Batman
Aquaman
Wonder Woman
Flash (Wally West)
Green Lantern (Hal Jordan)
Hawkman
Hawkgirl
Power Girl
Supergirl
Zatanna
Swamp Thing
Batgirl
Aqualad
Speedy
Black Canary
Dr.Fate
Vixen
Steel (John Henry Irons)
Atom Smasher
The Atom
Cyborg
Hawk & Dove
Nightwing
Hulk
Iron Man
Scarlet Witch
Black Widow
She-Hulk
The Vision
Thor
Wolverine
Storm
Cyclops
Rogue
Jean Grey
Spider-Man
Namor
Fantastic Four
Black Panther
Quicksilver
Dr. Strange
Silver Surfer
Captain America
VS.
Mario
Luigi
Peach
Wario
Samus Aran
Donkey Kong
Pit (Kid Icarus)
Link (Ocarina of Time)
Crash Bandicoot
Mega Man (Classic)
Pac-Man
Leon (Resident Evil)
Kirby
Diddy Kong
Banjo & Kazooie
Mumbo Jumbo (Banjo Kazooie)
Shantae
Earthworm Jim
Bayonetta
Battletoads
Cloud Strife (Final Fantasy 7)
Sonic the Hedgehog
Tails
Knuckles
Zero (Mega Man X)
Jak and Daxter
Sly Cooper
Bubsy (Bubsy game series)
Gex (Gex game series)
Crazy Dave with his Plants (Plants vs Zombies)
Dante (Devil May Cry)
Arthur (Ghosts 'n Goblins)
Ryu (Street Fighter)
Chun Li (Street Fighter)
Ken (Street Fighter)
Simon Belmont (Castlevania)
Rayman
Solid Snake
Parappa (PaRappa the Rapper)
Raiden (Metal Gear)
Ratchet and Clank
Sackboy (LittleBigPlanet)
Sir Daniel Fortesque (MediEvil)
Spike (Ape Escape)
So who would between Superheroes against game characters.
Note: DC comics Superheroes are the original Earth 52 characters and Marvels Superheroes are the original Earth 616 versions.