Screwball is fun, though I don't even remember the last time she was used, and Overdrive and Beetle under Spencer were great even if they're not as prevalent as other villains in terms of actually fighting Spider-Man.
Morlun was big in JMS' run, but he was successful as a villain moreso for his sheer strength, effectiveness, and power than his actual character. And I can't say there's much about the rest of his family that makes them standout...
Tiberius is at least a good villain in that I want nothing more than to see him get punched in the face, and PAD seems to be giving his character a lot more layers so there's definitely potential with him.
Ana is cool, and I think there would've been more with making her a real prominent villain in the books instead of just reviving Kraven considering how little has been done with either since Grim Hunt.
As for Lily...let's just say I think she looks better as the White Cat then she ever did as Menace
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Out of all the villains on the list I'd say Mr. Negative's gotten the most use, appearances, and prominence compared to the rest on the list, so he'd be the closest to being the "Greatest" of the bunch in my opinion.